Figure 10 | Scientific Reports

Figure 10

From: Design, synthesis, and discovery of novel oxindoles bearing 3-heterocycles as species-specific and combinatorial agents in eradicating Staphylococcus species

Figure 10

Characterization of protein affected by KS16. (A) Analysis of total cellular proteins from ATCC 25923 cells (1 × 109) treated with different concentrations of KS16. Total cellular proteins were resolved on 12% TGX stain-free gels and imaged. The ratio of A was calculated using Image Lab Software (Bio-Rad). One of the representative data from n = 3 was shown. (B) MALDI-TOF/MS spectrum of band A. Arrows indicate the peptide fragments obtained. (C) Identification of autolysin protein. Analysis of peptide fragments using Mascot and Swiss-Prot identified that peptides sequences are matching to autolysin protein derived from Staphylococcus aureus subsp. aureus CIGC341D. Amino acid sequences were taken from NCBIprot database (GenBank: EHT86028.1). Bold underlined sequences (29% coverage) represent peptides matched with autolysin. All images were processed in Adobe Illustrator CS6 (v16.0.0, Adobe Systems Inc., USA).

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