Figure 1 | Scientific Reports

Figure 1

From: Generation of new hair cells by DNA methyltransferase (Dnmt) inhibitor 5-azacytidine in a chemically-deafened mouse model

Figure 1

Loss of hair cell (HC) in chemically-damaged mature mouse inner ears. (a) Cochlear sections show HCs in normal hearing mice (arrows), whereas HCs are lost following chemical damage (arrowheads). (b) Quantitative study shows HC survival following chemical damage. In normal mouse cochlea, there are one row of IHCs and three rows of OHCs. Thus, the percentage of IHC survival is calculated by (number of IHCs/1) × 100%. The percentage of OHC survival is calculated by (number of OHCs/3) × 100%]. Scale bar: 50 µm. (c) The diagram shows the experimental design. The day of mice being deafened was set as experimental day 0. 5-aza or saline was injected into the mouse cochlea 3 days post deafening. Mice were followed up for 1–6 weeks. During one-week, two-week, four-week and six-week post-surgery, one group of animals were sacrificed, and their cochleae were cryosectioned to evaluate HCs. New HCs characterization and 5-aza concentration study were performed on animals two-week after 5-aza treatment.

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