Figure 7
Initial mechanistic study of new HCs regeneration. (a) No Ki67 staining is observed in cochlear sections of mice two weeks after receiving 5-aza injection (Ki67 positive control in Supplemental Data Fig. S1). Scale bar: 50 µm in cochlear section overview; 20 µm in organ of Corti (OC) highlight. (b) Relative Dnmt1 mRNA expression levels were measured in the saline- and 5-aza-injected deafened mice two weeks after injection. The results show decreased Dnmt1 mRNA expression in the 5-aza group compared to the saline group. (c) Relative mRNA expression of Notch and Wnt signaling genes in saline- and 5-aza-treated deafened mice two weeks after injection. It is observed that expression of Notch signaling genes Hes1 and Hey1 as well as Wnt signaling genes Fzd2 and Gsk3b are significantly downregulated, whereas Ctnnb1 was not affected. Scale bar: 50 µm.
