Figure 2

Sublytic injured podocytes attenuate immune complex deposition in subendothelial area in vivo. (a) Experimental schedule. LMB2① means 12 days after LMB2 exposure. LMB2② means 5 days after LMB2 exposure. (b) NEP25/hybridoma/PBS mice (n = 3) showed no abnormality in light microscopic study, while IgG and C3 deposits were observed along with capillary wall in immunofluorescence. Magnification, x400. Electron microscopy (EM) showed electron dense deposition only in the subendothelial area (arrow). Magnification, x700. (c) NEP25/hybridoma/LMB2 mice (12 days after LMB2 exposure, n = 3) showed fibrin deposition and epithelial cell hyperplasia without endocapillary proliferative lesions, similar to the findings in NEP25/LMB2 mice. Both IgG and C3 deposits were scant in the glomeruli, but were concentrated in the tubular lumen. NEP25/hybridoma/LMB2 (5 days after LMB2 exposure, n = 8) showed minor glomerular abnormalities without IC deposition in either glomeruli or tubular lumen. Magnification, x400 in the picture of glomerulus, or x100 in the picture of renal parenchyma. (d) Staining intensity score for IgG in glomeruli. NEP25/hybridoma/PBS mice showed significant increase of IgG deposition, whereas LMB2 treatment reduced the deposition in NEP25/hybridoma/LMB2 mice. NEP25/PBS (n = 3), NEP25/hybridoma/PBS (n = 3), and NEP25/hybridoma/LMB2 (5 days after LMB2 exposure, n = 8). *p < 0.05.