Figure 3 | Scientific Reports

Figure 3

From: Possible role of complement factor H in podocytes in clearing glomerular subendothelial immune complex deposits

Figure 3

Sublytic podocyte injury induces CFH in vivo. (a) qRT-PCR analysis of isolated glomeruli showed increased CFH expression in the setting of sublytic podocyte injury both with and without hybridoma 5 days after LMB2 exposure. The mRNA expression was compared in NEP25/PBS vs. NEP25/LMB2 (Day 5), NEP25/PBS vs. NEP25/hybridoma/PBS, NEP25/hybridoma/PBS vs. NEP25/hybridoma/LMB2 (Day 5), and NEP25/hybridoma/PBS vs NEP25/hybridoma/LMB2 (Day 12) mice. NEP25/PBS (n = 3), NEP25/LMB2 (Day 5, n = 3), NEP25/hybridoma/PBS (n = 3), NEP25/hybridoma/LMB2 (Day 5, n = 8), NEP25/hybridoma/LMB2 (Day 12, n = 3). *p < 0.05. (b) Immunofluorescence of CFH and podocin (podocyte marker) showed increased podocyte-derived CFH in the setting of sublytic podocyte injury. Magnification, x400. (c) Immunohistochemistry showed increased CFH staining in the position of podocyte in the setting of sublytic podocyte injury. Magnification, x400.

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