Figure 2
From: New PCR primers for metabarcoding environmental DNA from freshwater eels, genus Anguilla

Water sampling sites (yellow circles). The Karasugawa River (35.008°N, 139.085°E), Ito-Nakagawa River (35.005°N, 139.083°E), and Ito-Miyagawa River (35.002°N, 139.082°E; 35.003°N, 139.080°E) in Shizuoka and the Ebitorigawa River (35.550°N, 139.752°E) in Tokyo. The map overlay shows the sampling locations (yellow squares) within Shizuoka and Tokyo Prefectures within central Japan. Panels were made from imagery obtained from the Google Earth program.