Table 1 A list of primers for the 1st PCR. MiEel primers (universal primers of Anguilla for eDNA metabarcoding).
From: New PCR primers for metabarcoding environmental DNA from freshwater eels, genus Anguilla
Primers for the 1st-PCR | Sequence (5′-3′) |
|---|---|
MiEel (forward) | ACACTCTTTCCCTACACGACGCTCTTCCGATCT NNNNNNCTTACAGCAAACCTGACAGCAG |
MiEel (reverse) | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT NNNNNNTTGGTGTGCCATTATACGTTTTCTTG |
