Table 3 The number of reads with identify of more than 98.5% from eDNA samples from aquarium water in two tanks (Bibi, A. bicolor bicolor; Aust, A. australis sp.) and freshwater from the controlled pond and four rivers.

From: New PCR primers for metabarcoding environmental DNA from freshwater eels, genus Anguilla

Species

Number of sequence reads from two aquarium tanks, controlled pond and four natural rivers

Bibi tank

Aust tank

Controlled pond

Ebitorigawa

Karasugawa

Ito-Nakagawa

Ito-Miyagawa estuary

Ito-Miyagawa upstream

A. australis australis

0

16,360

0

0

0

0

0

0

A. australis schmidtii

0

0

0

0

0

0

0

0

A. bicolor bicolor

224

0

0

0

0

0

0

0

A. japonica

0

0

8,014

8

479

702

299

466

Total

224

16,360

8,014

8

479

702

299

466

  1. Out of 19 species and subspecies, four species detected from MiSeq sequencing were tabulated. Only A. japonica would be expected to be present the four rivers.