Table 3 The number of reads with identify of more than 98.5% from eDNA samples from aquarium water in two tanks (Bibi, A. bicolor bicolor; Aust, A. australis sp.) and freshwater from the controlled pond and four rivers.
From: New PCR primers for metabarcoding environmental DNA from freshwater eels, genus Anguilla
Species | Number of sequence reads from two aquarium tanks, controlled pond and four natural rivers | |||||||
---|---|---|---|---|---|---|---|---|
Bibi tank | Aust tank | Controlled pond | Ebitorigawa | Karasugawa | Ito-Nakagawa | Ito-Miyagawa estuary | Ito-Miyagawa upstream | |
A. australis australis | 0 | 16,360 | 0 | 0 | 0 | 0 | 0 | 0 |
A. australis schmidtii | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
A. bicolor bicolor | 224 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
A. japonica | 0 | 0 | 8,014 | 8 | 479 | 702 | 299 | 466 |
Total | 224 | 16,360 | 8,014 | 8 | 479 | 702 | 299 | 466 |