Figure 3

Classification of clusters formed in microwells. (a) Very tight clusters (b) tight clusters (c) loose clusters based on cell density using fluorescent intensity of DAPI (gray value). (d) Median gray values of clusters formed from each well of different cluster types (Very tight cluster n = 18 clusters; tight cluster n = 27 clusters; loose cluster n = 17 clusters). Data are presented as mean ± SEM (***p < 0.001) (Scale bar: 200 µm).