Figure 6

Effect of Ashwashila Treatment on Articular Cartilage Erosion of Ankle Joint. (A) Histological analysis of normal control (NC) animal ankle-joint stained with safranin ‘O’ show normal uncalcified cartilage (UC), calcified cartilage (CC), and subchondral bone (SB). (B) Ankle joint in disease control (DC) animal following treatment with C-Ab + LPS showed cartilage degradation extending up to SB. (C) Treatment of the diseased animal with Ashwashila (ASHW) limited the cartilage degradation till the UC region of the ankle-joint. (D) Following treatment of the diseased animals with Methotrexate (MTX) cartilage degradation was limited to UC. (E) Inflammatory lesion development was detected in the DC animals that showed significant reduction following treatment of the animals with ASHW or MTX. (F) Similar efficacy of ASHW and MTX was observed in anti-arthritic activity through reduction in lesion score as a function of percentage (%) inhibition. Values in the results are Mean ± SEM. A one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparison t-test was used to calculate the statistical difference. Student unpaired t-test was used to calculate the statistical difference in comparison to MTX (p-value # ≤ 0.05; ** ≤ 0.01).