Figure 6
From: CTLA-4 Protects against Angiotensin II-Induced Abdominal Aortic Aneurysm Formation in Mice
Overexpression of cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) suppresses immunoinflammatory responses in aneurysmal tissues. Seven days after the pump implantation, angiotensin II-infused apolipoprotein E-deficient (Apoe−/−) and CTLA-4-Tg/Apoe−/− mice were euthanized and total RNA was extracted from the supra-renal aortas. Messenger RNA expression of proinflammatory cytokines (interferon (IFN)-γ, interleukin (IL)-1β, IL-6), chemokine monocyte chemoattractant protein (MCP)-1, adhesion molecules (intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1), and matrix metalloproteinase (MMPs) (MMP-2, MMP-9) was quantified by quantitative real-time reverse transcription PCR and normalized to GAPDH. n = 4 to 6 per group. Data points represent individual animals. Horizontal bars represent means. Error bars indicate s.d. *P < 0.05; **P < 0.01; Mann-Whitney U-test. Ang-II, angiotensin II.
