Table 1 RNA integrity (quality) and concentrations obtained from non-irradiated and irradiated eggs before activation and non-irradiated and irradiated eggs after activation (Gyno 1n and Andro 1n, respectively).
From: Induced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs
Samples | TapeStation RIN | T-test p-value* | TapeStation Concentration | Qubit concentration | T-test p-value* | Nanodrop Concentration | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Group | Sample | RIN | Mean | SD | 175 Gy | 350 Gy | gyno 1n | andro 1n | ng/µl | Mean | SD | ng/µl | Mean | SD | 175 Gy | 350 Gy | gyno 1n | andro 1n | ng/µl | Mean | SD |
non-irradiated eggs | 0.1 | 8.7 | 8.6 | 0.21 | 0.0437 | 0.0060 | 0.0100 | 0.0266 | 64.8 | 61.2 | 4.8 | 74.9 | 70.7 | 14.4 | 0.9753 | 0.0652 | 0.0004 | 0.0002 | 85.7 | 86.8 | 18.3 |
0.2 | 8.6 | 54.1 | 52.3 | 67.1 | |||||||||||||||||
0.3 | 8.9 | 62.6 | 68.7 | 83.2 | |||||||||||||||||
0.4 | 8.4 | 63.3 | 86.9 | 111.3 | |||||||||||||||||
eggs irradiated with 175 Gy | 1.1 | 9.3 | 9.1 | 0.31 | x | 0.6832 | 0.6507 | 1 | 82.7 | 77.3 | 30.8 | 62.2 | 71.1 | 20.1 | x | 0.1188 | 0.0020 | 0.0012 | 81.6 | 88.2 | 17.4 |
1.2 | 8.7 | 47.4 | 52.6 | 73.7 | |||||||||||||||||
1.3 | 9.4 | 61 | 70.3 | 83.9 | |||||||||||||||||
1.4 | 9.1 | 118 | 99.3 | 113.5 | |||||||||||||||||
eggs irradiated with 350 Gy | 2.1 | 9.2 | 9.2 | 0.16 | x | 0.1340 | 0.6333 | 75.5 | 74.1 | 11.5 | 106 | 93.6 | 14.3 | x | 5E-05 | 3E-05 | 124.1 | 118.8 | 9.7 | ||
2.2 | 9.2 | 62.6 | 86.4 | 105.1 | |||||||||||||||||
2.3 | 9.4 | 68.8 | 105 | 127 | |||||||||||||||||
2.4 | 9 | 89.5 | 76.9 | 118.9 | |||||||||||||||||
Gyno 1n | 3.1 | 9 | 9.1 | 0.06 | x | 0.58 | 43.4 | 26.0 | 14.4 | 17.7 | 17.7 | 3.9 | x | 0.08 | 28.2 | 26.6 | 8.5 | ||||
3.2 | 9.1 | 22.1 | 17.5 | 22.5 | |||||||||||||||||
3.3 | 9 | 29.4 | 13 | 18.1 | |||||||||||||||||
3.4 | 9.1 | 9 | 22.5 | 37.7 | |||||||||||||||||
Andro 1n | 4.1 | 9.4 | 9.1 | 0.25 | x | 18 | 9.5 | 6.3 | 18.2 | 11.3 | 4.7 | x | 35.1 | 25.8 | 8 | ||||||
4.2 | 9.1 | 5.5 | 9.1 | 29.3 | |||||||||||||||||
4.3 | 9.2 | 10.6 | 10.1 | 17 | |||||||||||||||||
4.4 | 8.8 | 4 | 7.6 | 21.8 | |||||||||||||||||
