Figure 3 | Scientific Reports

Figure 3

From: Characterization of mouse ocular response to a 35-day spaceflight mission: Evidence of blood-retinal barrier disruption and ocular adaptations

Figure 3

Glial fibrillary acidic protein (GFAP) and aquaporin-4 (AQP-4) staining in the retina. (A) Representative micrographs of ocular sections after immunostaining with anti-GFAP and AQP-4 antibodies on flight (FLT) and ground control (GC) samples. AQP-4 positive staining is identified by green fluorescence, GFAP with red, and the cell nuclei with blue (DAPI). Increased AQP-4 staining was seen in the FLT group compared with controls. No significant differences in GFAP staining was noted between two groups. Scale bar = 50 μm. (B) The average fluorescence intensity for AQP-4 was measured and calculated using the ImageJ program. Fluorescence was averaged across 6 retinas per group. Values are represented as mean density ± SEM. *Significantly higher than ground control (GC) groups (p < 0.05).

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