Figure 5

FRAP analysis of sperm cells. (Panel A) Exemplificative gallery showing the fluorescence recovery after photobleaching (FRAP) technique. (Panel B) Graph representing the values of Calculated Diffusion Coefficient (CDC), expressed as cm2/sec × 10−9, of sperm samples treated with the different experimental treatments (various GO concentrations, BSA or MβCD). The box plot represent the mean, 25°–75°, 5–90° percentile and out-layers. (Panel C) Dose-dependence linking GO concentration to Calculated Diffusion Coefficient (CDC).