Table 1 PHB production and growth rate under different culture conditions and glutamate (Glu) supplies in the wild type (WT) and Δgdc mutant.
Nutrient condition | Glu supply (mM) | Maximum specific PHB production rate (mg PHB/g CDW/d) | Specific growth rate at exponential growth phase (d−1) | ||
|---|---|---|---|---|---|
WT | Δgdc | WT | Δgdc | ||
Normal | 0 | 3.0 ± 0.7 | 6.1 ± 0.7** | 0.36 ± 0.03 | 0.27 ± 0.03** |
10 | 3.0 ± 0.3 | 3.8 ± 0.3* | 0.35 ± 0.05 | 0.23 ± 0.04** | |
50 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.34 ± 0.04 | 0.25 ± 0.03** | |
Nitrogen deprivation | 0 | 4.3 ± 0.5 | 3.3 ± 0.5 | NA | NA |
10 | 3.1 ± 0.3 | 2.1 ± 0.2** | NA | NA | |
50 | 1.1 ± 0.1 | 0.0 ± 0.0** | NA | NA | |
Phosphorus deprivation | 0 | 7.3 ± 0.9 | 6.1 ± 1.0 | NA | NA |
10 | 5.7 ± 0.6 | 2.3 ± 0.8** | NA | NA | |
50 | 3.1 ± 0.3 | 3.1 ± 0.2 | NA | NA | |
