Figure 3
Glutamate evokes platelet spreading (under static condition) and aggregate/microthrombi formation (under arterial shear) on immobilized matrix. (A) Confocal images of phalloidin-FITC-labeled control and glutamate (500 µM)-treated platelets undergoing spreading upon immobilized fibrinogen under static condition in absence or presence of 100 µM CNQX as indicated. (B) Fluorescence microscopy images of calcein-stained control and glutamate (500 µM)-treated platelets adhered on to immobilized collagen under flow at shear rate 1500 s−1, in absence or presence of 100 µM CNQX as indicated. Figures are representative of images from 5 different fields of 3 independent experiments.
