Figure 6
Glutamate affects platelet mitochondrial function. (A,B) Measurement of mitochondrial transmembrane potential by MitoTracker Red in glutamate (500 µM)-treated platelets in presence or absence 100 µM CNQX. CCCP (100 µM) was employed as control to dissipate ΔΨ. (C,D) Glutamate-induced rise in intracellular ROS in absence or presence of CNQX as indicated. H2O2 (10 µM) was used as control to raise ROS in platelets. (E,F), polarograms exhibiting oxygen flux in human platelets stimulated either with thrombin (1 U/ml) or glutamate (500 µM). Blue line represents oxygen concentration within the chamber while red line traces rate of oxygen consumption by the cells. Bars represent atleast 5 independent experiments (mean ± SEM). *P < 0.05 as compared to resting platelets; #P < 0.05 as compared to glutamate-stimulated platelets.
