Table 1 Comparison of the optical properties of the dyes in solvents of different polarity.
From: Amino-isocyanoacridines: Novel, Tunable Solvatochromic Fluorophores as Physiological pH Probes
Hexane (Polarity index = 0.0) | Dioxane (Polarity index = 4.8) | Water (Polarity index = 9.0) | μe − μg/D (DFT calculation) | μe − μg/D (Lippert Mataga) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
λem,max (nm) | λex,max (nm) | Δν (cm−1) | λem,max (nm) | λex,max (nm) | Δν (cm−1) | λem,max (nm) | λex,max (nm) | Δν (cm−1) | ε (M−1cm−1) | ΦF (%) | Δλem (nm) | |||
AO* | 498 | 412 | 4191 | 500 | 426 | 3474 | 526 | 492 | 1313 | 44000 | 15 | 28 | 2a | 2.27a |
ICAAc | 503 | 418 | 4158 | 515 | 431 | 3784 | 553 | 466 | 3376 | 10132 | 1.8 | 53 | 6.1 | 5.6 ± 1.4 |
monoMICAAc | 491 | 425 | 3163 | 515 | 437 | 3466 | 554 | 472 | 3136 | 5196 | 2.9 | 63 | 6.8 | 8.1 ± 0.8 |
diMICAAc | 514 | 447 | 2916 | 539 | 456 | 3377 | 576 | 497 | 2760 | 13344 | 0.4 | 62 | 7.9 | 8.9 ± 1.1 |
