Table 3 Reproducibility of the spectroscopic pH sensing method using different buffers.
From: Amino-isocyanoacridines: Novel, Tunable Solvatochromic Fluorophores as Physiological pH Probes
A375/A359 | I376/I362 | pHSet | pHAbs | pHPL |
|---|---|---|---|---|
1.031 ± 0.003 1.043 ± 0.002 | 0.986 ± 0.006 1.005 ± 0.008 | 6.01 | 6.24 ± 0.06 5.98 ± 0.002 | 6.06 ± 0.03 5.97 ± 0.04 |
1.000 ± 0.002 1.020 ± 0.011 | 0.814 ± 0.004 0.857 ± 0.008 | 6.49 | 6.63 ± 0.01 6.42 ± 0.15 | 6.55 ± 0.01 6.45 ± 0.02 |
0.914 ± 0.003 0.934 ± 0.004 | 0.562 ± 0.002 0.623 ± 0.002 | 7.00 | 7.10 ± 0.01 7.02 ± 0.02 | 7.06 ± 0.00 6.94 ± 0.00 |
0.772 ± 0.011 0.751 ± 0.006 | 0.367 ± 0.019 0.365 ± 0.001 | 7.51 | 7.52 ± 0.03 7.57 ± 0.02 | 7.55 ± 0.07 7.56 ± 0.00 |
0.589 ± 0.006 0.565 ± 0.002 | 0.272 ± 0.001 0.267 ± 0.002 | 8.00 | 7.97 ± 0.02 8.03 ± 0.05 | 8.03 ± 0.01 8.08 ± 0.02 |
0.445 ± 0.009 0.440 ± 0.007 | 0.234 ± 0.001 0.231 ± 0.001 | 8.50 | 8.45 ± 0.04 8.47 ± 0.03 | 8.54 ± 0.03 8.60 ± 0.02 |
0.395 ± 0.002 0.377 ± 0.006 | 0.219 ± 0.002 0.219 ± 0.001 | 9.00 | 8.75 ± 0.02 8.91 ± 0.06 | 9.17 ± 0.23 9.19 ± 0.11 |
