Figure 2

Binding of levantetraose to endo-levanase E221A variant. Amino acids in the vicinity of the ligand are in turquoise and the levantetraose bound at four subsites is in magenta. The 2mFo-DFc electron density map of levantetraose contoured at 1σ is shown in grey (upper right). The residues of endo-levanase constituting the subsites of the ligand-binding pocket are listed on the right. LigPlot+61 was used for visualization of protein-ligand interactions.