Figure 7
Co-incubation of N- and C-terminal modules restores slight endo-levanase activity. Proteins were incubated with 5 g/L Ps_S levan and samples withdrawn at indicated time were spotted onto a TLC plate as follows. Lanes 1, 3 and 9 – samples of reaction with BT1760; lanes 2, 4 and 10 – samples of reaction with the 1:1 mixture of BT17601-349 and BT1760348-508; lanes 5 and 11 – respective samples of reaction with the 1:1 molecular ratio of BT17601-349 and BsCBM66; lanes 6 and 12 – samples of reaction with the N-terminal BT17601-349 module and lanes 7 and 13 with the C-terminal BT1760348-508. Lanes 8 and 14 shows samples from the reaction with BsCBM66. Lane 15 shows levan incubated during 24 hours under same conditions, but without the addition of enzymes or modules. The first and the last lane indicate the marker sugars: fructose (F), sucrose (S), 1-kestose (1-K), nystose (N) and levan (L). The scanned image is not digitally modified.
