Figure 2
Efferocytosis and TG-2 levels in MDMs. (a) MDMs were co-cultured with early apoptotic Jurkat T-cells for 30 minutes, and the uptake was assessed by flow cytometry. Data are expressed as the percentage of MDMs that had engulfed carboxy fluoroscein diacetate succinimidyl ester (CSFE)-stained Jurkat apoptotic cells, and they derive from independent cultures obtained from 25 healthy subjects and 50 CAD patients. (b) TG-2 was detected by western blot analysis. β-actin was used as a control of protein loading. Densitometry is shown in the bar graph. Data are expressed as mean ± SD, and they derive from MDMs obtained from 5 healthy subjects and 10 CAD patients. (c) Representative images (400× original magnification) and (d) quantitative analysis of TG-2 in round and spindle MDMs obtained from healthy subjects and CAD patients. Nuclei were visualized by Hoechst 33258. Data derive from independent cultures obtained from 10 healthy subjects and 30 CAD patients. *P < 0.05, **P < 0.005. Full blots are shown in Supplementary Fig. 1.
