Figure 4

TF levels and thrombin generation in MDMs. (a) TF was detected by western blotting. β-actin was used as a control of protein loading. Densitometry is shown in the bar graph. Data are expressed as mean ± SD and derive from MDMs obtained from 12 healthy subjects and 14 CAD patients. (b) Representative images (400× original magnification) of TF in MDMs obtained from healthy subjects and CAD patients. Nuclei were visualized by Hoechst 33258. Data derive from independent cultures obtained from 10 healthy subjects and 30 CAD patients. (c) Thrombin generation parameters measured in MDMs obtained from 16 healthy subjects and 20 CAD patients. (d) Quantitative analysis of TF levels in MDM morphotypes of healthy subjects and patients. Data derive from independent cultures obtained from 10 healthy subjects, 10 SA patients, 10 NSTEMI patients, and 10 STEMI patients. *P < 0.05, **P < 0.005. Full blots are shown in Supplementary Fig. 2.