Figure 2

PLAG1 Deficiency Affects Growth and Reproduction in Mice. (a) Observed and expected numbers of pup genotypes in litters from Plag1 heterozygote intercrosses. (b) Body weights of female and male pups at weaning (3-weeks-old). (c) Frequency of litters from breeding pairs of different genotypes maintained in continuous breeding for 90 days. Every breeding pair is represented with a horizontal line and the birth of a litter is illustrated with a circle. Quantification of the data is shown on the right. (d) Mean number of pups in litters from different parent genotypes. (e) Mean number of implanted embryos on 7.5–8.5 dpc. (f) Representative photos of uteri at dissection. Asterisks indicate implanted embryos. Scale bars are 1 cm. The data in b–e are presented as means + SEM and the number of observations is shown inside the columns. Statistical analysis by χ² test (a) or one-way ANOVA followed by Fisher LSD post-hoc test (b−f). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. F, female; HET, heterozygote; KO, knockout; M, male; WT, wildtype.