Figure 3
From: Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue
Experimental infection of intestinal slices cultured ex vivo. These microphotographs show the infection of the colonic mucosa with Entamoeba histolytica trophozoites in slices that were cultured for 3 h (a,b), 6 h (c,d), and 9 h (e,f). The most remarkable morphologic change is the ulceration of the mucosa as a consequence of the infection process; morphologically well conserved amoebae (arrows) are in close contact with epithelial cells (*) and are able to induce a severe acute inflammatory process composed mainly by neutrophils and eosinophils (dotted area in Fig. d). Figures b, d, and f correspond to microscopic magnifications of figures a, c, and e, respectively. H&E stain (Total magnification: 100X, 400X).
