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Figure 1

From: Human Sialic acid O-acetyl esterase (SIAE) – mediated changes in sensitivity to etoposide in a medulloblastoma cell line

Figure 1

Expression of GD3 acetylation pathway components in clinical MB samples. (A) Schematic of the GD3 acetylation pathway shows the roles of GD3 synthase, SIAE and CASD1 in the synthesis and turnover of GD3 and GD3A. (B) Expression of CASD1, encoding the enzyme that converts GD3 to GD3A is upregulated in MB samples (Pfister dataset) compared to non-neoplastic cerebellum (Roth dataset; p < 0.0001). (C) Expression of SIAE, encoding the enzyme which deacetylates GD3A to GD3 is significantly down-regulated in MB samples (Pfister dataset) compared to non-neoplastic cerebellum (Roth dataset; p = 0.0005). (D) Expression of CASD1 is highest in group 4 samples when compared to WNT and SHH (p < 0.0001), and group 3 (p = 0.0221). The expression of CASD1 is also significantly higher in group 3 compared to WNT (p = 0.00403). (E) Expression of SIAE, is significantly higher in WNT tumours compared to SHH (p < 0.0001), group 3 (p = 0.00043), and group 4 (p = 0.0031). Data obtained from the R2 database using the Northcott data set (gse21140). (F) The data from D and E analysed to generate a ratio of expression between CASD1 and SIAE for each sample. There is a significant inverse-correlation between CASD1 and SIAE expression in SHH, group 3, and group 4 subgroups when compared to WNT (SHH p = 0.0003; Group 3 p = −0.0008; Group 4 p < 0.0001). Data was analysed by (B and C) two-tailed unpaired t-test; (D–F) one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons post hoc test using graph pad prism 6 software. (*p < 0.05; **p < 0.01; ****p < 0.0001).

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