Figure 5
GD3 expression is increased with SIAE induction in RES256 cells. (A) Using flow cytometry expression of GD3A was found to be significantly reduced in EGFP expressing controls (p < 0.0180). No difference was seen in SIAE clones 1, 2 or S127A. (B) GD3 expression was significantly increased in SIAE clone 2 (p = 0.0171). No significant changes are seen in SIAE 1, S127A or EGFP expressing cells. SIAE expression was induced for 48 hours with doxycycline. Samples were analysed using a multi-parameter fluorescence-activated cell sorting (FACS) Calibur flow cytometer and CellQuest Pro software. Data analysed by one-way analysis of variance followed by Tukey’s multiple comparisons post-hoc test using Graph Pad (*p < 0.05; **p < 0.01) n = 3 ± SEM.
