Figure 2
From: LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
m1A profiling of RNAs in HIV-1 virion. (A) Scheme showing the preparation of the deep sequencing libraries of RNA isolated from HIV-1 virions. (B) Table showing the observed misincorporation (blue) or arrests (orange) of reverse transcriptase in the deep sequencing libraries. T stands for library Lib4 prepared with TGIRT reverse transcriptase, while Lib1-Lib3 libraries were prepared with SuperScriptTM III reverse transcriptase. (C) The percentages of the most abundant RNA reads in the deep sequencing libraries. (D) Table showing the extrapolated numbers of copies of RNAs in HIV-1 virion based on literature (∗)17,18 and on our LC/MS data.
