Figure 3
From: Enhancement of laccase activity by pre-incubation with organic solvents
Pre-incubation has advantages on enhancing DLac activity in the presence of the corresponding organic solvent. The organic-solvent tolerance of enzyme activity of DLac was investigated by adding acetone (a) or ethanol (b) into ABTS enzyme assay buffer. The 0% acetone (or 0% ethanol) pre-incubation condition in this experiment was laccase incubated in Tris buffer without any organic solvent. DLac activity without organic solvents during 1 hr pre-incubation and ABTS enzyme assay was defined as 1-fold. The significant difference (*) was set at P < 0.05 and the highly significant difference (**) was set at P < 0.001 in Student’s t-test, compared to their 0% organic solvent pre-incubation control. Data were shown as mean ± SD from at least three independent repeats.
