Figure 4
From: Enhancement of laccase activity by pre-incubation with organic solvents
Thermostability and 3-D structure of acetone-pre-incubated DLac. (a) Thermostability of DLac was decreased with increasing acetone concentration. Plots indicated the residual activities after 10 min of heat treatment. DLac activity after 10-min incubation at 25 °C was defined as 100%. Data were shown as mean ± SD from at least three independent repeats. (b) The secondary structure of DLac remained unchanged in acetone solution. Far-UV CD spectra were used to monitor DLac structure change in 0, 10, 30 and 50% acetone. (c) Crystals and 3-D structures of the tetragonal form of acetone-pre-incubated DLac and orthorhombic form of DLac were compared and superimposed. Four copper atoms are represented as orange spheres.
