Figure 5
From: Enhancement of laccase activity by pre-incubation with organic solvents

Organic-solvent enhancement of enzyme activity of general fungal laccases. Four purified laccases were pre-incubated with eight different solvents, and after 1 hr of pre-incubation, enzyme activity was measured by enzyme activity assay with ABTS used as a substrate. The enzyme activity of each laccase without organic solvent during pre-incubation was defined as 1-fold. The highly significant difference (**) was set at P < 0.001 in Student’s t-test, compared to their Tris buffer control.