Figure 7
From: Enhancement of laccase activity by pre-incubation with organic solvents
Acetone pre-incubation improve turnover rate and catalytic efficiency. The turnover rate (kcat, a), substrate-binding affinity (KM, b) and catalytic efficiency (kcat/KM, c) of laccases without or with acetone were measured. Four purified laccases were pre-incubated with acetone, and their enzyme activity was immediately measured by enzyme activity assay with ABTS used as a substrate at 30 °C. The significant difference (*) was set at P < 0.05 and the highly significant difference (**) was set at P < 0.001 in Student’s t-test, compared to their Tris buffer control.
