Figure 2 | Scientific Reports

Figure 2

From: Short exposure to cold atmospheric plasma induces senescence in human skin fibroblasts and adipose mesenchymal stromal cells

Figure 2

CAP He treatment leads to irreversible proliferation stop and cell cycle arrest. (A) ASC and dermal fibroblasts were treated (CAP or NT) and cell number was determined during a longer time-course (n = 3). (B) A time-course of cell numeration was performed on treated cells cultured 7 days and passaged once (n = 3). (C) Cell cycle analysis was performed by Propidium Iodide staining and flow cytometry to estimate the distribution in G0/G1 and G2/M 6 hours after treatment (ASC, n = 3; fibroblasts, n = 4). (D) The percentage of cells in S phase 24 hours after treatment was determined after EdU incorporation by flow cytometry (ASC, n = 3; fibroblasts, n = 4). Data represent mean ± SEM of independent experiments as indicated (n).

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