Table 1 Mitochondrial DNA molecule types observed in the transmission electron microscopic (TEM) analysis of parental HEK293T and MGME1 knockout (KO) cells, without or with 72 h ddC treatment.

From: Replication fork rescue in mammalian mitochondria

 

HEK untreated

HEK ddC

MGME1 KO untreated

MGME1 KO ddC

N

400

400

400

100

Catenated or recombining

9%

15%

11%

29%

Dimeric circles

0.1%

0.2%

1.3%

ND

Replicating

5%

8%

5%

19%

of which broken

10%

40%

57%

32%

  1. N = number of counted molecules. Note that catenated and recombining circles cannot be distinguished from the TEM imagery. Only 100 molecules were counted from the ddC treated MGME1 KO due to high levels of linear mtDNA in these samples and therefore rare dimeric molecules were not detected (ND).
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