Figure 2 | Scientific Reports

Figure 2

From: Coro2b, a podocyte protein downregulated in human diabetic nephropathy, is involved in the development of protamine sulphate-induced foot process effacement

Figure 2

Localization of Coro2b in human and mouse podocyte foot processes. (A,B) Immunofluorescent staining for Coro2b (green) and nephrin (red) or podocalyxin (red) in a transgenic tdtomato (blue) expressing podocyte mouse line imaged with confocal and stimulated emission depletion (STED) microscopy. Coro2b is detected in podocytes without any significant co-localization with nephrin, whereas overlapping signal with podocalyxin is observed. (C) Co-staining of Coro2b with epithelial cell membrane binding wheat germ agglutinin (WGA) in human podocytes, captured with STED microscopy. Coro2b is detected on the apical side of podocyte foot processes (D,E) Localization of Coro2b signal in mouse and human foot processes as detected by STED microscopy. The y-axis represents the relative localization of signal peak between the basal and apical membranes. Coro2b is dominantly detected at the apical side of mouse and human foot processes. Images were deconvoluted using SVI Huygen’s software (Hilversum, the Netherlands). Magnifications, A - 100x, B, C - 100x STED.

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