Figure 4
From: Screening by changes in stereotypical behavior during cell motility
Maximum caliber coupling strengths reflect behavioral differences. (A) Representative phase contrast images of AX2 (L) cells and mhcA− (R) cells. Scale bar is 10 μm. (B) Outlines of cells as they move for all conditions in the study. We imaged drug-treated cells paired with an AX2 parental control in differential interference contrast (DIC) and the mhcA− control with an AX2 parent in phase contrast microscopy, so we present these controls separately. Here, for clarity, we show outlines at intervals of one minute (or thirty frames). (C) Distributions in shape space of the BPB/LY treated cells (red) and the mhcA− strain (green) vs their respective controls (blue). Darker red and green areas show where the compared conditions overlap. (D) Lagrange multipliers λx = log10γx from MaxCal model trained on untreated cells (blue) and p-bromophenacyl bromide (BPB) and LY294002 treated cells (red). Untreated cells have coupling values for \({\lambda }_{\,1\,1}^{+-}\) and \({\lambda }_{\,2\,2}^{+-}\) much lower than 0, indicating the persistence in the direction of cell shape change (or more accurately, the rarity of reversals). In contrast, treatment with causes cells to have couplings values for \({\lambda }_{\,1\,1}^{+-}\) and \({\lambda }_{\,2\,2}^{+-}\) that are not significantly different to zero, indicating a lack of such persistence. Untreated cells also have much higher values than drug-treated for \({\lambda }_{\,1\,2}^{+-}\) and \({\lambda }_{\,1\,2}^{-+}\), indicating a stronger anticorrelation between the two components. Remaining distributions of rates can be found in Fig. S4. The distributions of all 14 rates differ significantly upon drug treatment α = 0.001 (Kolmogorov-Smirnov test). (E) The distributions for the same parameters for mhcA− cells (green) against their parental control (blue). The changes in \({\lambda }_{\,1\,1}^{+-}\), \({\lambda }_{\,1\,2}^{+-}\) and \({\lambda }_{\,1\,2}^{-+}\) show similar changes to those observed in drug treatment. These experiments used phase contrast microscopy rather than the DIC used in the drug-treated case, leading to the differences in the extremity of these coupling coefficients.
