Figure 7
Model of MSC reprogramming to pluripotency in different rigidity substrates. (A) Traction force, µ, as a function of substrate Young’s modulus, E, as given by equation 1. (B) Cumulative distribution of colony reprogramming, for soft (black points), intermediate (red squares) and rigid (blue stars) substrates, as a function of time. (C) Examples of typical simulations of colonies growing in substrates with different rigidities. The reprogrammed cells are represented in grey and the non-reprogrammed in red. The scale bars (on the first column) correspond to 100 µm. The simulation indicates that the reprogramming starts close to the colony center and moves quickly towards the periphery. Typically, the cell reprogramming within the same colony started later and was slower in stiffer substrates.
