Figure 10

Simplified spatial-temporal mouse embryo model. 3D embryo model based on hexagonal close-packing for embryos with 33 and 34 cells, and layer reconstruction of: (A) 33-cell and (B) 34-cell embryos. Packing of the: (C) 33-cell and (D) 34-cell embryos. (E) 5-cell and (F) 6-cell kernels formed of cells without external contacts. The transparent and solid spheres represent external and kernel cells, respectively. (G) Temporal representation of ICM cells from blastocysts at different number-of-cell stages and for different cell types. The gene expression of each cell is represented by a pie-chart of the expression of Pou5f1, Fgf4, Ctnnb1 and Dppa2. The gene expression is represented in log₂ scale. Higher gene expression corresponds to redder colour, and lower expression to bluer colour. The genes and their corresponding positions in the pie-chart are represented by the black and white pie-chart in the bottom. The semicircle of grey circles surrounding the top part of each group of single cells represents the blastocyst outer cells.