Figure 1
Effect of clindamycin on the levels of indigenous enterococci in the intestinal lumen and tissues, and translocation to organs in C57BL/6 mice. Each dot represents one mouse (n ≥ 7) and indicates the number of colony-forming units (CFUs) per gram of content (g of content) or per organ. The dotted line represents the detection limit. Horizontal bars represent the median values from two independent experiments. Enterococcal counts in the gut lumen (A) were conducted on luminal content from the small intestine (cSi), the caecum (cCA) and the colon (cCO) of C57BL/6J and C57BL/6N conventional mice that were untreated (◯) or treated for three consecutive days with clindamycin (●). Enterococcal counts in the gut tissues (B) were conducted on the small intestine (SI), the caecum (CA) and the colon (CO) from clindamycin-treated C57BL/6J and C57BL/6N conventional mice. Enterococcal counts in peripheral organs (C) were conducted on the mesenteric lymph nodes (MLNs), the mesenteric adipose tissue (MAT), the spleen (SP), the kidneys (KDs), the liver (LV) and the heart (HR) from clindamycin-treated C57BL/6J and C57BL/6N conventional mice. Statistical analysis was performed using the Mann–Whitney test. The asterisk (*) and hash (#) symbols indicate statistically significant differences between counts (*P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001); ns, non-significant difference.
