Figure 7
Oxidative stressors induce SGs in mammalian but not in zebrafish cells. (a,b) Representative confocal immunofluorescence for YB-1 (red) in (a) PAC2 cells and (b) HaCaT cells treated with 250 µM Na Ars, 300 µM H2O2 and 500 µM Cu(II) for 30 minutes. YB-1 positive SGs present in HaCaT cells are indicated by white arrows. (c,d) Quantification of aggregate size for the immunofluorescence analysis presented in panels a and b, respectively. (e) Cell viability assay (MTT) in control or YB-1-silenced PAC2 cells at 26 °C or after 300 μM H2O2 treatment for 45 minutes. Statistical analysis was performed using 1-way ANOVA followed by Tukey’s multiple comparisons test (see also Table S1).
