Figure 3 | Scientific Reports

Figure 3

From: Validation of monoclonal anti-PKC isozyme antibodies for flow cytometry analyses in human T cell subsets and expression in cord blood T cells

Figure 3

Expression of PKC isozymes in CD4+ and CD8+ T cells by flow cytometry analysis. Flow cytometry analysis of PKC isozyme expression in CD4+ and CD8+ T cells. (a) Representative histogram for PKC isozymes in whole blood assay gated on CD3+CD8− T-cells (left) and individual and mean values showing the variation in the population (n = 11) (right). (b) Representative histogram for PKC isozymes in whole blood assay gated on CD3+CD8+ T cells and values representing the variation in the population (n = 11). Dashed lines represent the isotype controls and solid lines represent anti-PKC isozyme antibody staining. Bar graphs show values for each individual and the mean ± SD expressed as change in median fluorescence intensity (ΔMFI) obtained after subtracting the isotype control MFI values from respective PKC isozyme MFI values.

Back to article page