Figure 6 | Scientific Reports

Figure 6

From: Validation of monoclonal anti-PKC isozyme antibodies for flow cytometry analyses in human T cell subsets and expression in cord blood T cells

Figure 6

Detection of PKC isozyme expression in mouse lymphocytes by Western blot and flow cytometry. Mouse spleens were removed and cells were separated. (a) Cell lysates were prepared for Western blot analysis for detection of PKC isozymes by using PKC isozymes specific antibodies as shown in figures, n = 1–2. The blots are presented individually for each staining, intact without splicing, with lanes in their entirety. (b) Murine whole blood or (c) splenic lymphocytes were stained for intracellular PKC isozymes expression as outlined in methods. Histogram data from murine lymphocyte population are shown. Shaded histogram represents isotype control, dashed line represents unstained population and solid line represents PKC isozymes. Quantitated data (mean ± SD) from mouse blood lymphocytes (n = 5) and mouse splenic cells (n = 2–5) are presented in the right hand panels.

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