Figure 7 | Scientific Reports

Figure 7

From: Validation of monoclonal anti-PKC isozyme antibodies for flow cytometry analyses in human T cell subsets and expression in cord blood T cells

Figure 7

Cord blood PKC isozyme expression in CD4+ and CD8+ T cells, monocytes and neutrophils. Whole blood from cord (n = 9) and adult donor blood (n = 4) were stained with anti-CD3 and anti-CD8 antibodies and co-stained for anti-PKC isozymes. The data are expressed as a percentage of cryopreserved adult donor PBMC that were analysed in parallel. PKC levels were quantified as change in median fluorescent intensity (ΔMFI) by flow cytometry and then expressed as percentage of cryopreserved adult control cells. (a) Expression of PKC isozymes in cord (open bars) and adult (shaded bars) blood CD4+ and CD8+ T cells. (b) Expression of PKC isozymes in cord blood monocytes and neutrophils as compared to adult control blood. These levels were quantified as change in ΔMFI after subtracting the MFI of respective isotype control by flow cytometry and shown as median. Data are presented as mean ± SD.

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