Figure 8 | Scientific Reports

Figure 8

From: Validation of monoclonal anti-PKC isozyme antibodies for flow cytometry analyses in human T cell subsets and expression in cord blood T cells

Figure 8

Kinetics of PKCζ and δ expression in cord blood CD3+CD8− and CD3+CD8+ T cells during maturation in culture. Cord blood T cell subsets expressing low levels of PKCζ at day-0 were matured in the presence of PHA (2 µg/ml), and IL-2 (10 ng/ml). Levels of the PKC isozymes were analysed by intracellular staining method as per method section at the indicated time point in (a) CD4+ and (b) CD8+ T cells during culture. ΔMFI of PKCs expressed as percentage of ΔMFI in cryopreserved PBMCs (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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