Figure 6 | Scientific Reports

Figure 6

From: Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells

Figure 6

Ability of the differentiated HLCs to store different metabolites. The resultant hiPSC-EB-HLCs and hiPSC-EB + EC-HLCs showed hepatocyte functional activities, such as (a) Indocyanine green (ICG - Cardiogreen) uptake; (b) ICG release after 6 hours; (c) cytoplasmic accumulation of neutral triglycerides and lipids indicated by Oil-Red O staining; (d) glycogen storage indicated by PAS staining; and (e) Acetylated low-density lipoprotein (DiI-ac-LDL) uptake in red. Both conditions with and without endothelial cells showed a similar ability for all the metabolites assessed. Undifferentiated hiPSCs were used as negative control. Scale bar 100 μm.

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