Figure 3
From: Reprogramming Escherichia coli for the production of prenylated indole diketopiperazine alkaloids
LC-MS/MS analysis of metabolite production by recombinant E. coli engineered for DMAPP/IPP production by the mevalonate pathway. SPE-treated culture supernatants of BL21AI bacteria carrying pJBEI-3085 and expressing CDPS74 (a), CDPS68 (b), or CDPS14 (c) plus various PTs, as indicated, were analysed. Each sample corresponded to 50 µl of culture supernatant. UV chromatograms recorded at 220 nm are shown between 6 and 30 min in (a,b) and between 11 and 35 min in (c). The scale of the Y-axis scale of the UV chromatograms was set from 0 to 700 mU. The major CDP synthesised by each CDPS is indicated in each panel with its MS characteristics. Bold Arabic numbers are placed above the peaks for which MS data are indicated.
