Table 2 Characterisation of the prenylated CDPs produced in E. coli.
From: Reprogramming Escherichia coli for the production of prenylated indole diketopiperazine alkaloids
Compound | Characterization | ||
|---|---|---|---|
# | Enzyme combination | Amount of lyophilised powder (culture volume) and purity of prenylated CDP† | NMR structure‡ |
4 | CDPS74 NotF | 17 mg (1 l); 95% |
|
5 | CDPS74 FtmPT1 | 26 mg (1 l); 95% |
|
6 | CDPS68 CdpC3PT | RT = 15.1 min§ 2 mg (800 ml); 74% |
|
7 | CDPS68 CdpC3PT | RT = 18.5 min§ 2.5 mg (800 ml); no contaminant detected |
|
8 | CDPS68 CdpC3PT | RT = 26.1 min§ 4.5 mg (800 ml); 95% |
|
9 | CDPS14 AnaPT | 6 mg (800 ml); 88% |
|
10, 11 | CDPS14 CTrpPT | 3.8 mg (450 ml); 82% |
|
