Figure 2
From: Loss of MafA and MafB expression promotes islet inflammation
MafA−/−MafB+/− islets have severe morphological changes and immune cell infiltration. (A–C) Histological images of 6-month-old wt and Maf mutant islets stained for amylase (red), insulin (green) and nucleus (DAPI; grey). (A,B) No amylase + exocrine cells were found inside the islets of wt and MafA−/− mice. (C) In MafA−/−MafB+/− islets amylase + cells were observed within the remnants of islets (dotted line indicate the border of an islet). (D) β cell area of wt, MafA−/− and MafA−/−MafB+/− is indicated in % pancreatic area. (E–H) MafA−/−MafB+/− islets were found next to the uncharacterized condensed cluster of cells, which were negative for (F) epithelial marker e-Cadherin (blue) but were (G,H) positive for CD45 and CD3 markers (red); scale bar is 40 µm. (I) Percentage of islets with clusters of CD3+ cells (>20 cells) in wt and Maf deficient mice. (J) CD3+ cell clusters (>50 cells) in direct contact with islets were only detected in MafA−/−MafB+/− pancreata. (K) CD3+ cells scattered/not in contact with islets were only observed in wt but not in MafA−/− and MafA−/−MafB+/− pancreata. CD3+ cells were counted in 5–7 animals per genotype. wt samples contain data points from wt and MafB+/− samples, no inflammation was observed in this genotype. Immunohistochemical analysis was performed in at least 3 replicates. Graphs are shown with mean ± SEM and data were analyzed with one-way ANOVA Tukey’s multiple comparison test with *P value ≤ 0.05; **P value ≤ 0.01 considered as significant.
