Figure 5
From: Loss of MafA and MafB expression promotes islet inflammation
MafA deletion in hematopoietic (MafAVAV) cells causes islet inflammation and reduction of β cell area. (A,B) MafA (red) expression was assessed in (A) wt and (B) MafAVAVMafB+/− islets stained with insulin (green) and DAPI for nucleus (grey). (C) Quantification of CD3 in MafAVAVMafB+/− pancreas (D,E). CD3+ T cells (red) and insulin (green) in wt (D) and MafAVAVMafB+/− (E). (F) Quantification of β cell area in MafAVAVMafB+/− pancreas. Representative images are from at least 3 experiments; N = 3–5 mice/genotype with scale bar of 20 µm. (E,F) Graphs are shown with mean ± SEM; N = 3–5 mice/genotype and are analyzed with (C) Tukey’s multiple comparison test and (F) unpaired T test with *P value ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 as significant.
