Figure 6
From: Loss of MafA and MafB expression promotes islet inflammation
CD4+ and CD8+ T cell activation profiles in pancreatic lymph nodes from Maf deficient mice. (A,B) Heatmap showing Fluidigm gene expression from 100 pooled CD4+ and CD8+ T cells from 6–8 months-old pancreatic lymph nodes (N = 4–8). (A,B) Heatmap color gradient scheme: dark red = highest expression; dark blue = lowest expression; white = intermediate; grey = no expression. (C–F) Flow cytometry analysis of 6–8 months-old pancreatic lymph nodes shows percentages of CD4+ and CD8+ T cells sub-classified into activated (CD44+ CD62L−), naïve (CD44−CD62L+), memory (CD44+CD62L+) and double negative (CD44−CD62L−) states. Results are combined from 4 individual experiments with a total of 3–6 mice/genotype. (C–F) Graphs are mean ± SEM and analyzed with two-way ANOVA Tukey’s multiple comparison tests with **P value ≤ 0.01 and ***P ≤ 0.001.
